Part:BBa_K1458002

ucp1 (Uncoupling protein 1)

ucp1 is the gene encoding UCP1 protein.

it is a Transmembrane protein naturally present in mitochondrias of brown adipose tissue. the gene itself is not encoded though in the mitochondrial genome but in the nuclear DNA, after translation it is taged and sent to the mitochondria. UCP1 increases the permeability of the inner mitochondrial membrane to protons, allowing them to move from the inner membrane space to the mitochondrial matrix, bypassing ATP synthase. This process weakens the coupling of the electron transport chain to the production of ATP. In an attempt to raise the pH gradient negatively affected by UCP1, a fast oxidation of substrates occurs while the synthesis rate of ATP stays low and oxidation energy is released as heat instead.

UCP1 is activated in the brown fat cell by fatty acids and inhibited by nucleotides.

1. Bugianesi, E., Moscatiello, S., Ciaravella, M.F. & Marchesini, G. (2010). Insulin resistance in nonalcoholic fatty liver disease. Curr Pharm Des 16: 1941-1951

2. Kozak, L.P. & Anunciado-Koza, R. (2008). UCP1: its involvement and utility in obesity. Int. J. Obes., 32 (Suppl. 7), pp. S32–S38

3. Wu, B.J., Kingston, R.E. & Morimoto, R.I. (1986). Human HSP70 promoter contains at least two distinct regulatory domains. Proc. Natl. Acad. Sci. USA 83: 629-633.

4. http://www.ncbi.nlm.nih.gov/gene/7350#reference-sequences

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 835
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 48